9th Annual WML Academic Symposium

Presenter(s): Philip Oji
Title: Development of an In Vitro Method using Fluorescent Resonance Energy Transfer to study Protein-Protein Interactions
Developed under the guidance of Dr. Stephen Sharkady , College of Pharmacy & Health Sciences

Fluorescent Resonance Energy Transfer (FRET) can be used to study Green fluorescent protein (GFP) and red fluorescent protein (RFP) joined together by the linker, Glycine-Glycine-Serine (GGS) repeated sequence, of various length. The first part of the project focused on creating the chimeric protein; GFP linked with RFP by 16 GGS amino acids repeated sequence (pET-GFP-L16-RFP). The resulting recombinant DNA was transformed into E. coli BL21/DE3 bacteria cells. Colony PCR of the DNA confirmed that the construction of clone, pET-GFP-L16-RFP, was successful.