4th Annual WML Academic Symposium

In this study the objective is to compare efficiency of two techniques-Dispersive micro Solid Phase Extraction (DµSPE) and Conventional Solid Phase Extraction (SPE) by using LC-MS. Esomeprazole is the S-enantiomer of omeprazole with an improved efficacy than omeprazole for the treatment of dyspepsia, gastroesophageal reflux disease (GORD/GERD), peptic ulcer disease (PUD) and Zollinger-Ellison syndrome. The primary use of esomeprazole is the treatment of gastrointestinal ulcers associated with Crohn’s disease. In this experiment, esomeprazole magnesium tablet and esomeprazole which is used as standard, are taken and will be subjected to undergo DµSPE and SPE techniques separately by using C-18 as a sorbent. The samples will be analyzed by using LC-MS with Electrospray Ionization (ESI) method. The method will be validated for Accuracy, Precision, Specificity, Stability, Limit of Detection (LOD) and Limit of Quantification (LOQ).