2nd Annual Academic Symposium - Andres Lam

3:00pm - 4:00pm
Main Lobby
Andres Lam
Study of SulfoNHS-LC-LC-Biotin for Cell Surface Protein Affinity Isolation and MS Characterization of Angiotensin II-(SS-Biotin)2

Developed under the guidance of
Dr. Sarah Liu
Pharmaceutical Sciences

In this project, the main goal is to study a non-cleavable biotin tag (SulfoNHS-LC-LC-biotin) as a tool to isolate cell surface proteins for LC-MS proteomics. The hypothesis is that with this biotin tag, high purity and high recovery isolation of tagged proteins can be achieved via Neutravidin affinity extraction by using LC-MS and gel-based techniques to optimize the biotinylation reaction, and loading/washing/eluting of Neutravidin affinity extraction. Angiotensin II will be used as a model peptide. Also, a previously detected angiotensin II-(SS-Biotin)2 will be characterized in more depth to understand where an unexpected secondary biotinylation occurred in the peptide sequence.